Polymerases & Mixes

Drug-resistance mutation frequency of SGA-derived HIV-1 polymerase genomes

Drug-resistance mutation frequency of SGA-derived HIV-1 polymerase genomes

mytaq

Drug-resistance mutation frequency of SGA-derived HIV-1 polymerase genomes within the CSF and plasma of HIV-1-infected people underneath non-suppressive remedy

HIV-1 evolution within the CSF and plasma could end in discordant drug-resistance mutations (DRMs) within the compartments. Single genome amplification (SGA) was used to generate partial HIV-1 polymerase genomes in paired CSF and plasma from 12 HIV-1 constructive CHARTER contributors categorized as neurocognitively unimpaired or with numerous levels of HIV-Related Neurocognitive Issues (HAND).

 

  • Topics had been viremic on mixture antiretroviral remedy (cART). HIV-1 DRMs and phylogenetic traits had been decided utilizing the Stanford HIVdb program and phylogenetic analyses. Particular person DRMs had been recognized extra continuously in plasma than in paired CSF (p=0.0078).
  • Vital variations within the ratios of DRMs in CSF and plasma had been present in Three people with HAND (3/7=43%). 2 HAND topics (2/7=29%) demonstrated one DRM in CSF not recognized in paired plasma.
  • Longitudinal analyses (n=4) revealed vital temporal variations within the ratios of DRMs within the compartments. Statistically vital variations within the frequency of DRMs within the CSF and plasma are readily present in these on non-suppressive cART.
  • Whereas compartment-based DRM discordance was largely in line with elevated drug-selective pressures within the plasma, overrepresentation of DRMs within the CNS can happen. Underlying mechanisms of HAND are advanced and multifactorial.
  • The scientific affect of DRM discordance on viral persistence and HAND pathogenesis stays unclear and warrants additional investigation in bigger, longitudinal cohorts.

    Significance: A number of antiretroviral brokers don’t effectively enter the CNS and impartial evolution of HIV-1 viral variants within the CNS and plasma can happen.

  • We use single genome amplification (SGA) in cross-sectional and longitudinal analyses to uniquely outline each the id and relative proportions of drug resistance mutations (DRMs) on particular person HIV-1 pol genomes within the cerebrospinal fluid (CSF) and plasma in people with incomplete viral suppression and recognized neurocognitive standing. Statistically vital variations within the ratio of DRMs within the CSF and plasma had been readily present in these on non-suppressive cART and overrepresentation of DRMs within the CNS can happen.
  • Though questions concerning the scientific significance of DRM discordance stay, within the quest for viral eradication, you will need to acknowledge {that a} vital, dynamic, compartment-based DRM ratio imbalance can exist, because it has the potential to go unnoticed within the setting of ordinary scientific drug resistance testing.
mytaq
mytaq

DNA Polymerase Iota (POLI) Antibody

abx036752-100ug 100 ug
EUR 469.2

DNA Polymerase Iota (POLI) Antibody

20-abx333843
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Iota (POLI) Antibody

20-abx323859
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

DNA Polymerase Iota (POLI) Antibody

abx236612-100ug 100 ug
EUR 577.2

DNA Polymerase iota Antibody / POLI

RQ4298 100 ug
EUR 419

Anti-DNA Polymerase iota (8G9)

YF-MA11349 100 ug
EUR 435.6
Description: Mouse monoclonal to DNA Polymerase iota

DNA polymerase iota (POLI) polyclonal antibody

ABP-PAB-11630 100 ug Ask for price

DNA Polymerase Iota (POLI) Antibody (HRP)

20-abx335403
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Iota (POLI) Antibody (FITC)

20-abx335404
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Iota (POLI) Antibody (Biotin)

20-abx335405
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-DNA Polymerase iota/POLI Antibody

A01376-1 100ug/vial
EUR 352.8

Human DNA Polymerase Iota (POLI) ELISA Kit

abx382346-96tests 96 tests
EUR 1093.2

Human DNA polymerase iota, POLI ELISA KIT

ELI-38100h 96 Tests
EUR 988.8

Mouse DNA polymerase iota, Poli ELISA KIT

ELI-12742m 96 Tests
EUR 1038

Anti-DNA pol iota Antibody

A01376 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for DNA pol iota Antibody (POLI) detection. Tested with WB in Human.

Anti-DNA pol iota antibody

STJ92742 200 µl
EUR 236.4
Description: Rabbit polyclonal to DNA pol iota.

DNA Polymerase

ABD3082 100 ug
EUR 525.6

DNA Polymerase

ABF5235 100 ug
EUR 525.6

DNA Polymerase alpha Antibody

DF3082 200ul
EUR 420

DNA Polymerase beta Antibody

DF6552 200ul
EUR 420

DNA Polymerase theta Antibody

DF13563 100ul
EUR 420

DNA Polymerase alpha Antibody

AF5235 200ul
EUR 420

DNA Polymerase beta Antibody

AF0148 200ul
EUR 420

DNA Polymerase beta antibody

70R-50226 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase beta antibody

DNA Polymerase lambda antibody

70R-32186 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase lambda antibody

DNA Polymerase zeta antibody

70R-33711 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase zeta antibody

70R-33727 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase theta antibody

70R-33737 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase theta antibody

DNA Polymerase beta antibody

70R-31161 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase beta antibody

DNA Polymerase alpha antibody

70R-31578 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase alpha antibody

DNA Polymerase beta Antibody

49605-100ul 100ul
EUR 399.6

DNA Polymerase beta Antibody

49605-50ul 50ul
EUR 286.8

DNA polymerase gamma antibody

70R-12532 100 ul
EUR 548.4
Description: Affinity purified Rabbit polyclonal DNA polymerase gamma antibody

DNA Polymerase gamma antibody

22870-100ul 100ul
EUR 468

DNA Polymerase ζ Antibody

33602-100ul 100ul
EUR 302.4

DNA Polymerase ζ Antibody

33602-50ul 50ul
EUR 224.4

DNA Polymerase θ Antibody

33611-100ul 100ul
EUR 302.4

DNA Polymerase θ Antibody

33611-50ul 50ul
EUR 224.4

DNA Polymerase α Antibody

33665-100ul 100ul
EUR 302.4

DNA Polymerase α Antibody

33665-50ul 50ul
EUR 224.4

DNA Polymerase beta Antibody

35404-100ul 100ul
EUR 468

DNA Polymerase λ Antibody

34098-100ul 100ul
EUR 302.4

DNA Polymerase λ Antibody

34098-50ul 50ul
EUR 224.4

DNA Polymerase β Antibody

33357-100ul 100ul
EUR 302.4

DNA Polymerase β Antibody

33357-50ul 50ul
EUR 224.4

DNA Polymerase beta Antibody

20-abx013060
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase alpha Antibody

20-abx013375
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase beta Antibody

20-abx008852
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase beta Antibody

ABF0148 100 ug
EUR 525.6

DNA polymerase beta Antibody

abx232432-100ug 100 ug
EUR 577.2

Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10201 500U
EUR 144
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10202 1000U
EUR 228
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

9001-2500
EUR 614.4

Taq DNA Polymerase

9001-500
EUR 229.2

PFU DNA Polymerase

9003-2500
EUR 614.4

PFU DNA Polymerase

9003-500
EUR 216

Laq? DNA Polymerase

9004-2500
EUR 732

Laq? DNA Polymerase

9004-500
EUR 261.6

DNA polymerase ? (Rat)

10-101 20ug
EUR 465.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 1107.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00203 500U
EUR 174
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Impact of sub-chronic publicity to cigarette smoke, digital cigarette and waterpipe on human lung epithelial barrier operate

Background: Making an allowance for a latest surge of a lung damage situation related to digital cigarette use, we devised an in vitro mannequin of sub-chronic publicity of human bronchial epithelial cells (HBECs) in air-liquid interface, to find out deterioration of epithelial cell barrier from sub-chronic publicity to cigarette smoke (CS), e-cigarette aerosol (EC), and tobacco waterpipe exposures (TW).

Strategies: Merchandise analyzed embrace commercially accessible e-liquid, with 0% or 1.2% focus of nicotine, tobacco mix (shisha), and reference-grade cigarette (3R4F). In a single set of experiments, HBECs had been uncovered to EC (Zero and 1.2%), CS or management air for 10 days utilizing 1 cigarette/day.

Within the second set of experiments, publicity of pseudostratified major epithelial tissue to TW or management air publicity was carried out 1-h/day, each different day, till Three exposures had been carried out.

After 16-18 h of final publicity, we investigated barrier operate/structural integrity of the epithelial monolayer with fluorescein isothiocyanate-dextran flux assay (FITC-Dextran), measurements of trans-electrical epithelial resistance (TEER), evaluation of the proportion of shifting cilia, cilia beat frequency (CBF), cell movement, and quantification of E-cadherin gene expression by reverse-transcription quantitative polymerase chain response (RT-qPCR).

Outcomes: When in comparison with air management, CS elevated fluorescence (FITC-Dextran assay) by 5.6 occasions, whereby CS and EC (1.2%) decreased TEER to 49 and 60% respectively. CS and EC (1.2%) publicity decreased CBF to 62 and 59%, and cilia shifting to 47 and 52%, respectively, when in comparison with management air. CS and EC (1.2%) elevated cell velocity in comparison with air management by 2.5 and a pair of.6 occasions, respectively.

The expression of E-cadherin decreased to 39% of management air ranges by CS publicity reveals an perception right into a believable molecular mechanism. Altogether, EC (0%) and TW exposures resulted in additional reasonable decreases in epithelial integrity, whereas EC (1.2%) considerably decreased airway epithelial barrier operate comparable with CS publicity.

Conclusions: The outcomes assist a poisonous impact of sub-chronic publicity to EC (1.2%) as evident by disruption of the bronchial epithelial cell barrier integrity, whereas additional analysis is required to handle the molecular mechanism of this statement in addition to TW and EC (0%) toxicity in persistent exposures.
Key phrases: Cigarette; Cilia; E-cadherin; E-cigarette; Nicotine; Tobacco; Waterpipe.

Anti-DNA Polymerase thet Antibody

A02205 100ul
EUR 476.4
Description: Rabbit Polyclonal DNA Polymerase thet Antibody. Validated in IHC and tested in Human.

Anti-Taq DNA Polymerase Antibody

48369-100ul 100ul
EUR 399.6

Anti-Taq DNA Polymerase Antibody

48369-50ul 50ul
EUR 286.8

Anti-DNA polymerase beta antibody

PAab02432 100 ug
EUR 426

anti- DNA polymerase beta antibody

FNab02432 100µg
EUR 606.3
Description: Antibody raised against DNA polymerase beta

Anti-DNA polymerase eta Antibody

STJ502747 100 µg
EUR 571.2

Anti-DNA polymerase theta antibody

STJ71548 100 µg
EUR 312

anti-DNA polymerase alpha

YF-PA24412 50 ul
EUR 400.8
Description: Mouse polyclonal to DNA polymerase alpha

anti-DNA polymerase beta

YF-PA24413 50 ul
EUR 400.8
Description: Mouse polyclonal to DNA polymerase beta

anti-DNA polymerase beta

YF-PA13864 50 ug
EUR 435.6
Description: Mouse polyclonal to DNA polymerase beta

anti-DNA polymerase beta

YF-PA13865 100 ul
EUR 483.6
Description: Rabbit polyclonal to DNA polymerase beta

anti-DNA polymerase eta

YF-PA13871 50 ug
EUR 435.6
Description: Mouse polyclonal to DNA polymerase eta

anti-DNA polymerase eta

YF-PA13872 100 ug
EUR 483.6
Description: Rabbit polyclonal to DNA polymerase eta

anti-DNA Polymerase epsilon

YF-PA19269 50 ul
EUR 435.6
Description: Mouse polyclonal to DNA Polymerase epsilon

anti-DNA Polymerase epsilon

YF-PA19270 50 ul
EUR 435.6
Description: Mouse polyclonal to DNA Polymerase epsilon

anti-DNA Polymerase epsilon

YF-PA19271 50 ug
EUR 435.6
Description: Mouse polyclonal to DNA Polymerase epsilon

anti-DNA Polymerase epsilon

YF-PA19272 100 ug
EUR 483.6
Description: Rabbit polyclonal to DNA Polymerase epsilon

anti-DNA Polymerase epsilon

YF-PA26240 50 ul
EUR 400.8
Description: Mouse polyclonal to DNA Polymerase epsilon

DNA Polymerase

ABF5235 100 ug
EUR 525.6

DNA Polymerase

ABD3082 100 ug
EUR 525.6

anti-DNA polymerase? antibody (rabbit antiserum)

70-041 50uL
EUR 496.8
Description: The anti-DNA polymerase? antibody (rabbit antiserum) is available in Europe and for worldwide shipping via Gentaur.

Anti-DNA Polymerase iota/POLI Antibody

A01376-1 100ug/vial
EUR 352.8

Anti-POLK/Dna Polymerase Kappa Antibody

A01987 100ug
EUR 518.4
Description: Goat Polyclonal POLK/Dna Polymerase Kappa Antibody. Validated in WB and tested in Human.

Anti-DNA Polymerase lambda/POLL Antibody

A00959-2 100ug/vial
EUR 352.8

Anti-Taq DNA Polymerase Conjugated Antibody

C48369 100ul
EUR 476.4

Anti-DNA Polymerase beta Monoclonal Antibody

M01946 100ug
EUR 476.4
Description: Rabbit Monoclonal DNA Polymerase beta Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat.

Anti-DNA Polymerase beta/POLB Antibody

PB9812 100ug/vial
EUR 352.8

Anti-DNA polymerase eta/POLH Antibody

PB9813 100ug/vial
EUR 352.8

Anti-DNA polymerase eta Antibody BIOTIN

STJ502748 100 µg
EUR 703.2

Anti-DNA polymerase eta Antibody FITC

STJ502749 100 µg
EUR 703.2

anti-DNA Polymerase epsilon p59

YF-PA24414 50 ul
EUR 400.8
Description: Mouse polyclonal to DNA Polymerase epsilon p59

anti-DNA polymerase delta p50

YF-PA13866 50 ug
EUR 435.6
Description: Mouse polyclonal to DNA polymerase delta p50

anti-DNA polymerase delta p50

YF-PA13867 100 ug
EUR 483.6
Description: Rabbit polyclonal to DNA polymerase delta p50

Anti-DNA Polymerase theta (1C11)

YF-MA17459 100 ug
EUR 435.6
Description: Mouse monoclonal to DNA Polymerase theta

anti-DNA Polymerase Kappa/POLK

YF-PA19042 50 ul
EUR 435.6
Description: Mouse polyclonal to DNA Polymerase Kappa/POLK

anti-DNA Polymerase Kappa/POLK

YF-PA19043 50 ug
EUR 435.6
Description: Mouse polyclonal to DNA Polymerase Kappa/POLK

anti-DNA Polymerase Kappa/POLK

YF-PA19044 100 ug
EUR 483.6
Description: Rabbit polyclonal to DNA Polymerase Kappa/POLK

Anti-DNA polymerase alpha (3C11)

YF-MA14797 100 ug
EUR 435.6
Description: Mouse monoclonal to DNA polymerase alpha

anti-DNA Polymerase Kappa/POLK

YF-PA26181 50 ul
EUR 400.8
Description: Mouse polyclonal to DNA Polymerase Kappa/POLK

Anti-DNA Polymerase iota (8G9)

YF-MA11349 100 ug
EUR 435.6
Description: Mouse monoclonal to DNA Polymerase iota

DNA polymerase gamma antibody

70R-12532 100 ul
EUR 548.4
Description: Affinity purified Rabbit polyclonal DNA polymerase gamma antibody

DNA Polymerase beta antibody

70R-50226 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase beta antibody

DNA Polymerase lambda antibody

70R-32186 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase lambda antibody

DNA Polymerase zeta antibody

70R-33711 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase zeta antibody

70R-33727 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase theta antibody

70R-33737 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase theta antibody

DNA Polymerase beta antibody

70R-31161 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase beta antibody

DNA Polymerase alpha antibody

70R-31578 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase alpha antibody

DNA Polymerase beta Antibody

49605-100ul 100ul
EUR 399.6

DNA Polymerase beta Antibody

49605-50ul 50ul
EUR 286.8

DNA Polymerase beta Antibody

35404-100ul 100ul
EUR 468

DNA Polymerase λ Antibody

34098-100ul 100ul
EUR 302.4

DNA Polymerase λ Antibody

34098-50ul 50ul
EUR 224.4

DNA Polymerase ζ Antibody

33602-100ul 100ul
EUR 302.4

DNA Polymerase ζ Antibody

33602-50ul 50ul
EUR 224.4

DNA Polymerase θ Antibody

33611-100ul 100ul
EUR 302.4

DNA Polymerase θ Antibody

33611-50ul 50ul
EUR 224.4

DNA Polymerase β Antibody

33357-100ul 100ul
EUR 302.4

DNA Polymerase β Antibody

33357-50ul 50ul
EUR 224.4

DNA Polymerase gamma antibody

22870-100ul 100ul
EUR 468

DNA Polymerase beta Antibody

20-abx008852
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase beta Antibody

20-abx013060
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase alpha Antibody

20-abx013375
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase beta Antibody

ABF0148 100 ug
EUR 525.6

DNA polymerase beta Antibody

abx232432-100ug 100 ug
EUR 577.2

DNA Polymerase beta Antibody

DF6552 200ul
EUR 420

DNA Polymerase alpha Antibody

DF3082 200ul
EUR 420

DNA Polymerase theta Antibody

DF13563 100ul
EUR 420

DNA Polymerase alpha Antibody

AF5235 200ul
EUR 420

DNA Polymerase beta Antibody

AF0148 200ul
EUR 420

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 418.8

DNA pol α Polyclonal Antibody

40844-100ul 100ul
EUR 302.4

DNA pol α Polyclonal Antibody

40844-50ul 50ul
EUR 224.4

DNA polymerase ? (Rat)

10-101 20ug
EUR 465.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 1107.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase

9001-2500
EUR 614.4

Taq DNA Polymerase

9001-500
EUR 229.2

PFU DNA Polymerase

9003-2500
EUR 614.4

PFU DNA Polymerase

9003-500
EUR 216

Laq? DNA Polymerase

9004-2500
EUR 732

Laq? DNA Polymerase

9004-500
EUR 261.6

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00203 500U
EUR 174
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00204 1000U
EUR 286.8
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00205 2500U
EUR 626.4
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Leave a Reply

Your email address will not be published.