Polymerases & Mixes

Drug-resistance mutation frequency of SGA-derived HIV-1 polymerase genomes

Drug-resistance mutation frequency of SGA-derived HIV-1 polymerase genomes

mytaq

Drug-resistance mutation frequency of SGA-derived HIV-1 polymerase genomes within the CSF and plasma of HIV-1-infected people underneath non-suppressive remedy

HIV-1 evolution within the CSF and plasma could end in discordant drug-resistance mutations (DRMs) within the compartments. Single genome amplification (SGA) was used to generate partial HIV-1 polymerase genomes in paired CSF and plasma from 12 HIV-1 constructive CHARTER contributors categorized as neurocognitively unimpaired or with numerous levels of HIV-Related Neurocognitive Issues (HAND).

 

  • Topics had been viremic on mixture antiretroviral remedy (cART). HIV-1 DRMs and phylogenetic traits had been decided utilizing the Stanford HIVdb program and phylogenetic analyses. Particular person DRMs had been recognized extra continuously in plasma than in paired CSF (p=0.0078).
  • Vital variations within the ratios of DRMs in CSF and plasma had been present in Three people with HAND (3/7=43%). 2 HAND topics (2/7=29%) demonstrated one DRM in CSF not recognized in paired plasma.
  • Longitudinal analyses (n=4) revealed vital temporal variations within the ratios of DRMs within the compartments. Statistically vital variations within the frequency of DRMs within the CSF and plasma are readily present in these on non-suppressive cART.
  • Whereas compartment-based DRM discordance was largely in line with elevated drug-selective pressures within the plasma, overrepresentation of DRMs within the CNS can happen. Underlying mechanisms of HAND are advanced and multifactorial.
  • The scientific affect of DRM discordance on viral persistence and HAND pathogenesis stays unclear and warrants additional investigation in bigger, longitudinal cohorts.

    Significance: A number of antiretroviral brokers don’t effectively enter the CNS and impartial evolution of HIV-1 viral variants within the CNS and plasma can happen.

  • We use single genome amplification (SGA) in cross-sectional and longitudinal analyses to uniquely outline each the id and relative proportions of drug resistance mutations (DRMs) on particular person HIV-1 pol genomes within the cerebrospinal fluid (CSF) and plasma in people with incomplete viral suppression and recognized neurocognitive standing. Statistically vital variations within the ratio of DRMs within the CSF and plasma had been readily present in these on non-suppressive cART and overrepresentation of DRMs within the CNS can happen.
  • Though questions concerning the scientific significance of DRM discordance stay, within the quest for viral eradication, you will need to acknowledge {that a} vital, dynamic, compartment-based DRM ratio imbalance can exist, because it has the potential to go unnoticed within the setting of ordinary scientific drug resistance testing.
mytaq
mytaq

DNA Polymerase Iota (POLI) Antibody

abx028115-400ul 400 ul
EUR 523

DNA Polymerase Iota (POLI) Antibody

abx028115-80l 80 µl
EUR 286

DNA Polymerase Iota (POLI) Antibody

abx236612-100ug 100 ug
EUR 481

DNA Polymerase Iota (POLI) Antibody

20-abx333843
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-DNA Polymerase iota (8G9)

YF-MA11349 100 ug
EUR 363
Description: Mouse monoclonal to DNA Polymerase iota

Anti-DNA Polymerase iota/POLI Antibody

A01376-1 100ug/vial
EUR 294

DNA Polymerase Iota (POLI) Antibody (HRP)

20-abx335403
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Iota (POLI) Antibody (FITC)

20-abx335404
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Iota (POLI) Antibody (Biotin)

20-abx335405
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA polymerase iota (POLI) polyclonal antibody

ABP-PAB-11630 100 ug Ask for price

Human DNA Polymerase Iota (POLI) ELISA Kit

abx382346-96tests 96 tests
EUR 911

Human DNA polymerase iota, POLI ELISA KIT

ELI-38100h 96 Tests
EUR 824

Mouse DNA polymerase iota, Poli ELISA KIT

ELI-12742m 96 Tests
EUR 865

Anti-DNA pol iota Antibody

A01376 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for DNA pol iota Antibody (POLI) detection. Tested with WB in Human.

Anti-DNA pol iota antibody

STJ92742 200 µl
EUR 197
Description: Rabbit polyclonal to DNA pol iota.

DNA Polymerase

ABF5235 100 ug
EUR 438

DNA Polymerase

ABD3082 100 ug
EUR 438

DNA Polymerase gamma antibody

22870-100ul 100ul
EUR 390

DNA Polymerase beta Antibody

35404-100ul 100ul
EUR 390

DNA Polymerase β Antibody

33357-100ul 100ul
EUR 252

DNA Polymerase β Antibody

33357-50ul 50ul
EUR 187

DNA Polymerase ζ Antibody

33602-100ul 100ul
EUR 252

DNA Polymerase ζ Antibody

33602-50ul 50ul
EUR 187

DNA Polymerase θ Antibody

33611-100ul 100ul
EUR 252

DNA Polymerase θ Antibody

33611-50ul 50ul
EUR 187

DNA Polymerase α Antibody

33665-100ul 100ul
EUR 252

DNA Polymerase α Antibody

33665-50ul 50ul
EUR 187

DNA Polymerase λ Antibody

34098-100ul 100ul
EUR 252

DNA Polymerase λ Antibody

34098-50ul 50ul
EUR 187

DNA Polymerase beta Antibody

49605-100ul 100ul
EUR 333

DNA Polymerase beta Antibody

49605-50ul 50ul
EUR 239

DNA Polymerase beta Antibody

20-abx008852
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase beta Antibody

20-abx013060
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase alpha Antibody

20-abx013375
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA polymerase beta Antibody

abx232432-100ug 100 ug
EUR 481

DNA Polymerase alpha Antibody

AF5235 200ul
EUR 304
Description: DNA Polymerase α Antibody detects endogenous levels of total DNA Polymerase α.

DNA Polymerase beta Antibody

ABF0148 100 ug
EUR 438

DNA Polymerase alpha Antibody

DF3082 200ul
EUR 304
Description: DNA Polymerase α Antibody detects endogenous levels of total DNA Polymerase α.

DNA Polymerase beta Antibody

AF0148 200ul
EUR 304
Description: DNA Polymerase beta antibody detects endogenous levels of total DNA Polymerase beta.

DNA Polymerase beta antibody

70R-31161 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase beta antibody

DNA Polymerase alpha antibody

70R-31578 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase alpha antibody

DNA Polymerase lambda antibody

70R-32186 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase lambda antibody

DNA Polymerase zeta antibody

70R-33711 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase zeta antibody

70R-33727 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase theta antibody

70R-33737 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase theta antibody

DNA polymerase gamma antibody

70R-12532 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal DNA polymerase gamma antibody

DNA Polymerase beta antibody

70R-50226 100 ul
EUR 244
Description: Purified Polyclonal DNA Polymerase beta antibody

DNA Polymerase theta Antibody

DF13563 100ul
EUR 350

PKC Iota (PKC iota) Antibody

abx033120-400ul 400 ul
EUR 523

PKC Iota (PKC iota) Antibody

abx033120-80l 80 µl
EUR 286

PKC Iota (PKC iota) Antibody

abx236486-100ug 100 ug
EUR 481

Taq DNA Polymerase

BA00103 500U
EUR 87
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

S116 1x250 units
EUR 76

Pfu DNA Polymerase

S117 2x250 units
EUR 94

Pfu DNA Polymerase

S118 10x250 units
EUR 303

Bst DNA Polymerase

S600 2000 U
EUR 107

DNA polymerase ? (Rat)

10-101 20ug
EUR 388
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 923
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

BIOTAQ DNA Polymerase

BIO-21040 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21046 250 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21047 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21048 5000 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21051 250 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21052 500 Units Ask for price

BIOTAQ DNA Polymerase

BIO-21060 2500 Units Ask for price

MangoTaq DNA Polymerase

BIO-21078 5000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21082 2000 Units Ask for price

Impact of sub-chronic publicity to cigarette smoke, digital cigarette and waterpipe on human lung epithelial barrier operate

Background: Making an allowance for a latest surge of a lung damage situation related to digital cigarette use, we devised an in vitro mannequin of sub-chronic publicity of human bronchial epithelial cells (HBECs) in air-liquid interface, to find out deterioration of epithelial cell barrier from sub-chronic publicity to cigarette smoke (CS), e-cigarette aerosol (EC), and tobacco waterpipe exposures (TW).

Strategies: Merchandise analyzed embrace commercially accessible e-liquid, with 0% or 1.2% focus of nicotine, tobacco mix (shisha), and reference-grade cigarette (3R4F). In a single set of experiments, HBECs had been uncovered to EC (Zero and 1.2%), CS or management air for 10 days utilizing 1 cigarette/day.

Within the second set of experiments, publicity of pseudostratified major epithelial tissue to TW or management air publicity was carried out 1-h/day, each different day, till Three exposures had been carried out.

After 16-18 h of final publicity, we investigated barrier operate/structural integrity of the epithelial monolayer with fluorescein isothiocyanate-dextran flux assay (FITC-Dextran), measurements of trans-electrical epithelial resistance (TEER), evaluation of the proportion of shifting cilia, cilia beat frequency (CBF), cell movement, and quantification of E-cadherin gene expression by reverse-transcription quantitative polymerase chain response (RT-qPCR).

Outcomes: When in comparison with air management, CS elevated fluorescence (FITC-Dextran assay) by 5.6 occasions, whereby CS and EC (1.2%) decreased TEER to 49 and 60% respectively. CS and EC (1.2%) publicity decreased CBF to 62 and 59%, and cilia shifting to 47 and 52%, respectively, when in comparison with management air. CS and EC (1.2%) elevated cell velocity in comparison with air management by 2.5 and a pair of.6 occasions, respectively.

The expression of E-cadherin decreased to 39% of management air ranges by CS publicity reveals an perception right into a believable molecular mechanism. Altogether, EC (0%) and TW exposures resulted in additional reasonable decreases in epithelial integrity, whereas EC (1.2%) considerably decreased airway epithelial barrier operate comparable with CS publicity.

Conclusions: The outcomes assist a poisonous impact of sub-chronic publicity to EC (1.2%) as evident by disruption of the bronchial epithelial cell barrier integrity, whereas additional analysis is required to handle the molecular mechanism of this statement in addition to TW and EC (0%) toxicity in persistent exposures.
Key phrases: Cigarette; Cilia; E-cadherin; E-cigarette; Nicotine; Tobacco; Waterpipe.

Anti-DNA polymerase eta Antibody

STJ502747 100 µg
EUR 476

Anti-DNA Polymerase thet Antibody

A02205 100ul
EUR 397
Description: Rabbit Polyclonal DNA Polymerase thet Antibody. Validated in IHC and tested in Human.

Anti-DNA polymerase theta antibody

STJ71548 100 µg
EUR 260

anti- DNA polymerase beta antibody

FNab02432 100µg
EUR 505.25
Description: Antibody raised against DNA polymerase beta

Anti-Taq DNA Polymerase Antibody

48369-100ul 100ul
EUR 333

Anti-Taq DNA Polymerase Antibody

48369-50ul 50ul
EUR 239

Anti-DNA polymerase beta antibody

PAab02432 100 ug
EUR 355

anti-DNA polymerase beta

YF-PA13864 50 ug
EUR 363
Description: Mouse polyclonal to DNA polymerase beta

anti-DNA polymerase beta

YF-PA13865 100 ul
EUR 403
Description: Rabbit polyclonal to DNA polymerase beta

anti-DNA polymerase eta

YF-PA13871 50 ug
EUR 363
Description: Mouse polyclonal to DNA polymerase eta

anti-DNA polymerase eta

YF-PA13872 100 ug
EUR 403
Description: Rabbit polyclonal to DNA polymerase eta

anti-DNA polymerase alpha

YF-PA24412 50 ul
EUR 334
Description: Mouse polyclonal to DNA polymerase alpha

anti-DNA polymerase beta

YF-PA24413 50 ul
EUR 334
Description: Mouse polyclonal to DNA polymerase beta

anti-DNA Polymerase epsilon

YF-PA26240 50 ul
EUR 334
Description: Mouse polyclonal to DNA Polymerase epsilon

anti-DNA Polymerase epsilon

YF-PA19269 50 ul
EUR 363
Description: Mouse polyclonal to DNA Polymerase epsilon

anti-DNA Polymerase epsilon

YF-PA19270 50 ul
EUR 363
Description: Mouse polyclonal to DNA Polymerase epsilon

anti-DNA Polymerase epsilon

YF-PA19271 50 ug
EUR 363
Description: Mouse polyclonal to DNA Polymerase epsilon

anti-DNA Polymerase epsilon

YF-PA19272 100 ug
EUR 403
Description: Rabbit polyclonal to DNA Polymerase epsilon

Anti-DNA Polymerase beta Monoclonal Antibody

M01946 100ug
EUR 397
Description: Rabbit Monoclonal DNA Polymerase beta Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat.

Anti-DNA Polymerase beta/POLB Antibody

PB9812 100ug/vial
EUR 294

Anti-DNA polymerase eta/POLH Antibody

PB9813 100ug/vial
EUR 294

Anti-DNA polymerase eta Antibody BIOTIN

STJ502748 100 µg
EUR 586

Anti-DNA polymerase eta Antibody FITC

STJ502749 100 µg
EUR 586

anti-DNA polymerase? antibody (rabbit antiserum)

70-041 50uL
EUR 414
Description: The anti-DNA polymerase? antibody (rabbit antiserum) is available in Europe and for worldwide shipping via Gentaur.

Anti-DNA Polymerase lambda/POLL Antibody

A00959-2 100ug/vial
EUR 294

Anti-DNA Polymerase iota/POLI Antibody

A01376-1 100ug/vial
EUR 294

Anti-POLK/Dna Polymerase Kappa Antibody

A01987 100ug
EUR 432
Description: Goat Polyclonal POLK/Dna Polymerase Kappa Antibody. Validated in WB and tested in Human.

Anti-Taq DNA Polymerase Conjugated Antibody

C48369 100ul
EUR 397

DNA Polymerase

ABF5235 100 ug
EUR 438

DNA Polymerase

ABD3082 100 ug
EUR 438

Anti-DNA Polymerase iota (8G9)

YF-MA11349 100 ug
EUR 363
Description: Mouse monoclonal to DNA Polymerase iota

Anti-DNA polymerase alpha (3C11)

YF-MA14797 100 ug
EUR 363
Description: Mouse monoclonal to DNA polymerase alpha

anti-DNA polymerase delta p50

YF-PA13866 50 ug
EUR 363
Description: Mouse polyclonal to DNA polymerase delta p50

anti-DNA polymerase delta p50

YF-PA13867 100 ug
EUR 403
Description: Rabbit polyclonal to DNA polymerase delta p50

Anti-DNA Polymerase theta (1C11)

YF-MA17459 100 ug
EUR 363
Description: Mouse monoclonal to DNA Polymerase theta

anti-DNA Polymerase epsilon p59

YF-PA24414 50 ul
EUR 334
Description: Mouse polyclonal to DNA Polymerase epsilon p59

anti-DNA Polymerase Kappa/POLK

YF-PA26181 50 ul
EUR 334
Description: Mouse polyclonal to DNA Polymerase Kappa/POLK

anti-DNA Polymerase Kappa/POLK

YF-PA19042 50 ul
EUR 363
Description: Mouse polyclonal to DNA Polymerase Kappa/POLK

anti-DNA Polymerase Kappa/POLK

YF-PA19043 50 ug
EUR 363
Description: Mouse polyclonal to DNA Polymerase Kappa/POLK

anti-DNA Polymerase Kappa/POLK

YF-PA19044 100 ug
EUR 403
Description: Rabbit polyclonal to DNA Polymerase Kappa/POLK

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

DNA Polymerase gamma antibody

22870-100ul 100ul
EUR 390

DNA Polymerase beta Antibody

35404-100ul 100ul
EUR 390

DNA Polymerase β Antibody

33357-100ul 100ul
EUR 252

DNA Polymerase β Antibody

33357-50ul 50ul
EUR 187

DNA Polymerase ζ Antibody

33602-100ul 100ul
EUR 252

DNA Polymerase ζ Antibody

33602-50ul 50ul
EUR 187

DNA Polymerase θ Antibody

33611-100ul 100ul
EUR 252

DNA Polymerase θ Antibody

33611-50ul 50ul
EUR 187

DNA Polymerase λ Antibody

34098-100ul 100ul
EUR 252

DNA Polymerase λ Antibody

34098-50ul 50ul
EUR 187

DNA Polymerase beta Antibody

49605-100ul 100ul
EUR 333

DNA Polymerase beta Antibody

49605-50ul 50ul
EUR 239

DNA Polymerase beta Antibody

20-abx008852
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase beta Antibody

20-abx013060
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase alpha Antibody

20-abx013375
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA polymerase beta Antibody

abx232432-100ug 100 ug
EUR 481

DNA Polymerase alpha Antibody

AF5235 200ul
EUR 304
Description: DNA Polymerase α Antibody detects endogenous levels of total DNA Polymerase α.

DNA Polymerase beta Antibody

ABF0148 100 ug
EUR 438

DNA Polymerase alpha Antibody

DF3082 200ul
EUR 304
Description: DNA Polymerase α Antibody detects endogenous levels of total DNA Polymerase α.

DNA Polymerase beta Antibody

AF0148 200ul
EUR 304
Description: DNA Polymerase beta antibody detects endogenous levels of total DNA Polymerase beta.

DNA Polymerase beta antibody

70R-31161 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase beta antibody

DNA Polymerase alpha antibody

70R-31578 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase alpha antibody

DNA Polymerase lambda antibody

70R-32186 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase lambda antibody

DNA Polymerase zeta antibody

70R-33711 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase zeta antibody

70R-33727 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase theta antibody

70R-33737 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase theta antibody

DNA polymerase gamma antibody

70R-12532 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal DNA polymerase gamma antibody

DNA Polymerase beta antibody

70R-50226 100 ul
EUR 244
Description: Purified Polyclonal DNA Polymerase beta antibody

DNA Polymerase theta Antibody

DF13563 100ul
EUR 350

DNA pol α Polyclonal Antibody

40844-100ul 100ul
EUR 252

DNA pol α Polyclonal Antibody

40844-50ul 50ul
EUR 187

Anti-DNA Polymerase Kappa/POLK (6F2)

YF-MA11510 100 ug
EUR 363
Description: Mouse monoclonal to DNA Polymerase Kappa/POLK

Anti-DNA Polymerase Kappa/POLK (4F12)

YF-MA18501 100 ug
EUR 363
Description: Mouse monoclonal to DNA Polymerase Kappa/POLK

Anti-DNA Polymerase epsilon p59 (1A3)

YF-MA14798 100 ug
EUR 363
Description: Mouse monoclonal to DNA Polymerase epsilon p59

Taq DNA Polymerase

BA00103 500U
EUR 87
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

S116 1x250 units
EUR 76

Pfu DNA Polymerase

S117 2x250 units
EUR 94

Pfu DNA Polymerase

S118 10x250 units
EUR 303

Bst DNA Polymerase

S600 2000 U
EUR 107

DNA polymerase ? (Rat)

10-101 20ug
EUR 388
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 923
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

BIOTAQ DNA Polymerase

BIO-21040 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21046 250 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21047 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21048 5000 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21051 250 Units Ask for price

Leave a Reply

Your email address will not be published.