Polymerases & Mixes

DNA Polymerase Facilitating Error-Prone DNA Damage Tolerance in Crenarchaeota

DNA Polymerase Facilitating Error-Prone DNA Damage Tolerance in Crenarchaeota

mytaq

A Distinctive B-Household DNA Polymerase Facilitating Error-Susceptible DNA Harm Tolerance in Crenarchaeota

Sulfolobus islandicus codes for 4 DNA polymerases: three are of the B-family (Dpo1, Dpo2, and Dpo3), and one is of the Y-family (Dpo4). Western evaluation revealed that among the many 4 polymerases, solely Dpo2 exhibited DNA damage-inducible expression.
To research how these DNA polymerases might contribute to DNA harm tolerance in S. islandicus, we carried out genetic evaluation of their encoding genes on this archaeon. Plasmid-borne gene expression revealed that Dpo2 will increase cell survival upon DNA harm on the expense of mutagenesis. Gene deletion research confirmed though dpo1 is important, the remaining three genes are dispensable. Moreover, though Dpo4 features in housekeeping translesion DNA synthesis (TLS), Dpo2, a B-family DNA polymerase as soon as predicted to be inactive, features as a damage-inducible TLS enzyme solely liable for focused mutagenesis, facilitating GC to AT/TA conversions within the course of.
Collectively, our knowledge point out that Dpo2 is the principle DNA polymerase liable for DNA harm tolerance and is the first supply of focused mutagenesis. Provided that crenarchaea encoding a Dpo2 even have a low-GC composition genome, the Dpo2-dependent DNA restore pathway could also be conserved on this archaeal lineage.
Key phrases: Crenarchaeota; DNA harm tolerance; DNA polymerase; Dpo2; Dpo4; Sulfolobus islandicus; genetic evaluation.

A Vital Appraisal and Suggestions for Price-Effectiveness Research of Poly(ADP-Ribose) Polymerase Inhibitors in Superior Ovarian Most cancers

Background: Ovarian most cancers is the fifth main reason behind most cancers loss of life in ladies within the US. With poly(ADP-ribose) polymerase (PARP) inhibitors having proven promising ends in ongoing trials, there may be curiosity in higher understanding their financial worth.
Goal: This research aimed to evaluate and consider the standard of printed cost-effectiveness analyses (CEAs), and supply suggestions for CEAs on this setting.
Strategies: A scientific literature evaluate of the MEDLINE and EMBASE databases was carried out in June 2019 to establish CEAs of PARP inhibitors in treating superior ovarian most cancers from peer-reviewed journals and conferences. Key data from the recognized publications have been extracted and reviewed. The standard of full-text research was assessed utilizing the High quality of Well being Financial Research instrument. Suggestions for future CEAs have been developed based mostly on the findings from the literature evaluate.
Outcomes: Eighteen CEAs (5 in full texts) met the inclusion standards. Most adopted a US healthcare or societal perspective. Nearly all of the research didn’t clearly show the financial mannequin construction. No research reported the validation of mannequin projections based mostly on inner or exterior knowledge.
Surrogate outcomes equivalent to incremental prices per progression-free life-year gained have been the most typical outcomes reported. Nearly all of research drew their conclusions based mostly on surrogate outcomes, even with no theoretical or empirical threshold for price effectiveness. All 5 full-text research included some kind of sensitivity or state of affairs analyses. The important thing drivers of the incremental cost-effectiveness ratio have been therapy length, results, and prices, well being utility, and prevalence of BRCA mutations.
Conclusion: Within the current CEAs for PARP inhibitors, there have been uncertainties and challenges resulting in variation in high quality. We offered suggestions to enhance consistency and high quality of CEAs on this setting, which is able to assist to higher perceive the worth of PARP inhibitors, enhance choice making, and cut back potential misallocation of sources.
mytaq
mytaq

DNA Polymerase beta Antibody

49605-100ul 100ul
EUR 399.6

DNA Polymerase beta Antibody

49605-50ul 50ul
EUR 286.8

DNA Polymerase beta antibody

70R-31161 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase beta antibody

DNA Polymerase beta antibody

70R-50226 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase beta antibody

DNA Polymerase beta Antibody

35404-100ul 100ul
EUR 468

DNA Polymerase beta Antibody

ABF0148 100 ug
EUR 525.6

DNA Polymerase beta Antibody

20-abx008852
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase beta Antibody

20-abx013060
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase beta Antibody

DF6552 100ul
EUR 280
Description: Human,Mouse,Rat

DNA Polymerase beta Antibody

AF0148 100ul
EUR 280
Description: Human,Mouse,Rat

DNA Polymerase beta Antibody

AF0148-100ul 100ul
EUR 280

DNA Polymerase beta Antibody

AF0148-200ul 200ul
EUR 350

DNA Polymerase beta Antibody

E18-0148-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

DNA Polymerase beta Antibody

E18-0148-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase beta Antibody

DF6552-100ul 100ul
EUR 280

DNA Polymerase beta Antibody

DF6552-200ul 200ul
EUR 350

DNA Polymerase beta Antibody / POLB

RQ5162 100ul
EUR 356.15
Description: The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. [RefSeq]

DNA polymerase beta Antibody / POLB

R31898 100 ug
EUR 356.15
Description: Polymerase (DNA directed), beta, also known as POLB, is an enzyme that, in humans, is encoded by the POLB gene. It is localized on 8p11.2. The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. It is found that a truncated POLB is expressed in primary colorectal tumors and inhibits the normal repair function of wildtype POLB. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. Additionally, human POLB forms a complex with and is methylated by PRMT6. In vitro, methylated POLB possesses significantly higher DNA polymerase activity when compared to that of unmodified enzyme. The increase in DNA polymerase activity upon methylation is due to the enhanced DNA binding and processivity of POLB.

Human DNA Polymerase beta Antibody

33292-05111 150 ug
EUR 313.2

Anti-DNA polymerase beta antibody

PAab02432 100 ug
EUR 426

DNA Polymerase beta Conjugated Antibody

C49605 100ul
EUR 476.4

Rabbit anti-DNA Polymerase beta Antibody

YLD2552-100ul 100 ul
EUR 320
Description: Rabbit polyclonal antibody to DNA Polymerase beta

Rabbit anti-DNA Polymerase beta Antibody

YLD2552-50ul 50 ul
EUR 200
Description: Rabbit polyclonal antibody to DNA Polymerase beta

Rabbit anti-DNA Polymerase beta Antibody

DL90907A-100ul 100 ul
EUR 342
Description: DNA polymerase beta

Rabbit anti-DNA Polymerase beta Antibody

DL90907A-50ul 50 ul
EUR 239.4
Description: DNA polymerase beta

Monoclonal Anti-Rat DNA polymerase beta antibody # 1

DNPB11-M 100 ug
EUR 578.4

Polymerase (Dna Directed), beta Antibody

20-abx114589
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Anti-DNA Polymerase beta/POLB Antibody

PB9812 100ug/vial
EUR 352.8

anti-DNA polymerase beta

YF-PA24413 50 ul
EUR 400.8
Description: Mouse polyclonal to DNA polymerase beta

anti-DNA polymerase beta

YF-PA13864 50 ug
EUR 435.6
Description: Mouse polyclonal to DNA polymerase beta

anti-DNA polymerase beta

YF-PA13865 100 ul
EUR 483.6
Description: Rabbit polyclonal to DNA polymerase beta

Anti-DNA Polymerase beta Antibody [JM93-12]

ET1705-12 100ul
EUR 231
Description: DNA polymerase beta, also known as POLB, is an enzyme present in eukaryotes. In humans, it is encoded by the POLB gene. In eukaryotic cells, DNA polymerase beta (POLB) performs base excision repair (BER) required for DNA maintenance, replication, recombination, and drug resistance. The mitochondrial DNA of mammalian cells is constantly under attack from oxygen radicals released during ATP production. Mammalian cell mitochondria contain an efficient base excision repair system employing POLB that removes some frequent oxidative DNA damages. POLB thus has a key role in maintaining the stability of the mitochondrial genome. An analysis of the fidelity of DNA replication by polymerase beta in the neurons from young and very aged mice indicated that aging has no significant effect on the fidelity of DNA synthesis by polymerase beta. This finding was considered to provide evidence against the error catastrophe theory of aging.

DNA Polymerase beta

E8ET1705-12 100ul
EUR 275
Description: Available in various conjugation types.

Anti-DNA Polymerase beta Monoclonal Antibody

M01946 100ug
EUR 476.4
Description: Rabbit Monoclonal DNA Polymerase beta Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat.

Human DNA Polymerase beta Antibody (Biotin Conjugate)

33292-05121 150 ug
EUR 442.8

DNA Polymerase beta recombinant monoclonal antibody

A5473 100ul X 3
EUR 714
Description: A recombinant monoclonal antibody from rabbit against human DNA Polymerase beta for WB,ELISA

Polymerase DNA Directed Beta (POLb) Antibody

20-abx174135
  • EUR 1028.40
  • EUR 526.80
  • 1 mg
  • 200 ug

Polymerase DNA Directed Beta (POLb) Antibody

20-abx178070
  • EUR 1446.00
  • EUR 693.60
  • 1 mg
  • 200 ug

Polymerase DNA Directed Beta (POLB) Antibody

abx332418-100ul 100 ul
EUR 510

Polymerase DNA Directed Beta (POLB) Antibody

abx037131-100ug 100 ug
EUR 469.2

Polymerase DNA Directed Beta (POLB) Antibody

20-abx328340
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Polymerase DNA Directed Beta (POLB) Antibody

20-abx001412
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Polymerase DNA Directed Beta (POLB) Antibody

20-abx304879
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Polymerase DNA Directed Beta (POLB) Antibody (HRP)

20-abx304880
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Polymerase DNA Directed Beta (POLB) Antibody (FITC)

20-abx304881
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Human DNA Polymerase beta AssayLite Antibody (RPE Conjugate)

33292-05151 150 ug
EUR 513.6

Human DNA Polymerase beta AssayLite Antibody (APC Conjugate)

33292-05161 150 ug
EUR 513.6

Human DNA Polymerase beta AssayLite Antibody (FITC Conjugate)

33292-05141 150 ug
EUR 513.6

Polymerase DNA Directed Beta (POLB) Antibody (Biotin)

20-abx304882
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Human DNA Polymerase beta AssayLite Antibody (PerCP Conjugate)

33292-05171 150 ug
EUR 565.2

DNA Polymerase beta Rabbit mAb

A2412-100ul 100 ul
EUR 492

DNA Polymerase beta Rabbit mAb

A2412-200ul 200 ul
EUR 685.2

DNA Polymerase beta Rabbit mAb

A2412-20ul 20 ul
EUR 265.2

DNA Polymerase beta Rabbit mAb

A2412-50ul 50 ul
EUR 344.4

DNA Polymerase beta Rabbit pAb

E2382035 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase beta Rabbit mAb

A2412 100μL
EUR 778.7
Description: Recombinant fusion protein containing a sequence corresponding to amino acids 8200-8400 of human Nesprin 1 (Q8NF91).

DNA Polymerase Antibody

E11-11836C 100μg
EUR 225
Description: Available in various conjugation types.

Anti-Taq DNA Polymerase Antibody

48369-100ul 100ul
EUR 399.6

Anti-Taq DNA Polymerase Antibody

48369-50ul 50ul
EUR 286.8

Anti-Taq DNA Polymerase Antibody

E8EM1701-49 100ul
EUR 275
Description: Available in various conjugation types.

DNA Polymerase beta Blocking Peptide

20-abx063101
  • EUR 326.40
  • EUR 493.20
  • 1 mg
  • 5 mg

DNA Polymerase beta Blocking Peptide

AF0148-BP 1mg
EUR 234

DNA Polymerase beta Recombinant Protein

91-139 0.05 mg
EUR 651.3
Description: Human DNA polymerase beta is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase beta .

DNA Polymerase beta Cell ELISA Kit

abx595180-96tests 96 tests
EUR 764.4

DNA polymerase beta ELISA KIT|Human

EF009144 96 Tests
EUR 826.8

DNA Polymerase λ Antibody

34098-100ul 100ul
EUR 302.4

DNA Polymerase λ Antibody

34098-50ul 50ul
EUR 224.4

DNA Polymerase β Antibody

33357-100ul 100ul
EUR 302.4

DNA Polymerase β Antibody

33357-50ul 50ul
EUR 224.4

DNA Polymerase ζ Antibody

33602-100ul 100ul
EUR 302.4

DNA Polymerase ζ Antibody

33602-50ul 50ul
EUR 224.4

DNA Polymerase θ Antibody

33611-100ul 100ul
EUR 302.4

DNA Polymerase θ Antibody

33611-50ul 50ul
EUR 224.4

DNA Polymerase α Antibody

33665-100ul 100ul
EUR 302.4

DNA Polymerase α Antibody

33665-50ul 50ul
EUR 224.4

DNA Polymerase β Antibody

E11-0173C 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase ζ Antibody

E11-10144C 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase θ Antibody

E11-10173C 100μg
EUR 225
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E11-10322C 100μg
EUR 225
Description: Available in various conjugation types.

DNA Polymerase ζ Antibody

E033602 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase θ Antibody

E033611 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E033665 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase λ Antibody

E034098 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase β Antibody

E033357 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E18-5235-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E18-5235-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E19-3082 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Anti-Taq DNA Polymerase Antibody [3C3]

EM1701-49 100ul
EUR 210
Description: Polymerase Chain Reaction (PCR), invented by Kary B. Mullis, at the Cetus Corporation, who was awarded the 1993 Nobel Prize for chemistry for PCR, is a technique to exponentially amplify in vitro a small quantity of a specific nucleotide sequence using a thermostable (Taq) DNA polymerase. Anti-Taq Antibody is an ideal tool for hot-start PCR with Taq DNA polymerase. The Anti-Taq Antibody binds to Taq DNA polymerase and arrests the activity of Taq DNA Polymerase, preventing non-specific and primer dimer amplification resulted from non-specific priming at ambient temperature for the duration of time prior to PCR thermal cycling. During the initial denaturing step in PCR thermal cycling, the Anti-Taq Antibody is denatured and the Taq DNA polymerase is then released, thus regaining its full DNA polymerase activity. The result indicates that anti-Taq DNA Polymerase antibody increases the specificity and sensitivity of the PCR.

DNA Polymerase zeta antibody

70R-33711 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase zeta antibody

70R-33727 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase zeta Antibody

E38PA4807 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase iota Antibody

E38PA5564 100ul
EUR 225
Description: Available in various conjugation types.

Anti-DNA Polymerase Antibody

E38A6546 100ug/100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase gamma antibody

22870-100ul 100ul
EUR 468

DNA polymerase gamma antibody

70R-12532 100 ul
EUR 548.4
Description: Affinity purified Rabbit polyclonal DNA polymerase gamma antibody

DNA Polymerase alpha antibody

70R-31578 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase alpha antibody

DNA Polymerase theta antibody

70R-33737 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase theta antibody

Multiplex polymerase chain response for genetically modified potato occasion AV43-6-G7 quantification. Proof of effectivity

One of many methods to enhance the laboratory management methodology of genetically modified organisms of plant origin (GMO) is to make use of multiplexing – an strategy that lets you improve the variety of targets and enlarge the variety of concurrently processed samples, maximizing the potential of polymerase chain response in actual time (PCR-RT).
 The purpose of the research is to develop a quantitative identification protocol for genetically engineered (GE) potato occasion AV43-6-G7 within the format of duplex PCR-RT with the usage of TaqMan® PCR know-how.
 Materials and strategies. The duplex system included 2 forms of particular DNA-primers and fluorescence-labeled probes: the primary one is for detection of transformation occasion AV43- 6-G7 DNA sequence, the second is for detection of Stp23 taxon-specific potato gene.
PCR parameters have been chosen by empirical number of concentrations of primers and probes, Mg2+ ions, deoxyribonucleotides, stabilizing agent for polymerase, in addition to primer annealing temperature and incubation length for every stage of the cycle.
 Outcomes. On account of these research, the composition of the response combination was optimized for the detection and quantification of GE potato occasion AV43-6-G7 in meals. Oligonucleotide primers and fluorescent probes have been chosen.
The compositions of response mixtures and temperature-time parameters of PCR have been examined: 2.5-fold response buffer for PCR-RT within the presence of ROX (carboxy-X-rhodamine), particular to the GE element primers (AV43-6-G7-f/AV43-6-G7-r) and goal taxon (GRF3/ GRR3) at 300 nM/300 nM and 100 nM/100 nM, probes at 200 nM and 200 nM, respectively; bovine serum albumin – 0.04%; MgCl2 – 3.5 mM, deoxynucleoside triphosphates – 0.Three mM, in addition to the temperature-time profile of the response (preliminary denaturation of 95 °C – 5 min, adopted by 45 cycles: 95 °С – 20 sec, 58 °С – 20 sec, 62 °С – 40 sec).
 Conclusion. The validity of the developed methodology is confirmed by laboratory research and testifies to its reliability.
Key phrases: GM-potato; PCR-RT; duplex PCR; strategies of management; transformation eventAV43-6-G7

DNA Polymerase alpha 1 Antibody

E38PA1906 100ul
EUR 225
Description: Available in various conjugation types.

Monoclonal Anti-Rat DNA polymerase beta antibody # 1

DNPB11-M 100 ug
EUR 578.4

Monoclonal Anti-Human DNA Polymerase gamma antibody # 1

DNPG13-M 100 ug
EUR 578.4

Monoclonal Anti-Human DNA Polymerase epsilon antibody # 1

DNPE12-M 100 ug
EUR 578.4

anti-DNA polymerase alpha

YF-PA24412 50 ul
EUR 400.8
Description: Mouse polyclonal to DNA polymerase alpha

Rabbit anti-DNA Polymerase alpha 1 Antibody

DL90906A-100ul 100 ul
EUR 270
Description: POLA; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180

Rabbit anti-DNA Polymerase alpha 1 Antibody

DL90906A-50ul 50 ul
EUR 189
Description: POLA; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180

Rabbit anti-DNA Polymerase alpha 1 Antibody

YLD2551-100ul 100 ul
EUR 320
Description: Rabbit polyclonal antibody to DNA Polymerase alpha 1

Rabbit anti-DNA Polymerase alpha 1 Antibody

YLD2551-50ul 50 ul
EUR 200
Description: Rabbit polyclonal antibody to DNA Polymerase alpha 1

POLA2 Antibody / DNA polymerase alpha subunit B

RQ6788 100ug
EUR 356.15
Description: DNA polymerase alpha subunit 2/B is an enzyme that in humans is encoded by the POLA2 gene. POLA2 may play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity).

DNA Polymerase alpha Antibody / POLA1 (C-Terminal Region)

F54188-0.05ML 0.05 ml
EUR 140.25
Description: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

DNA Polymerase alpha Antibody / POLA1 (C-Terminal Region)

F54188-0.2ML 0.2 ml
EUR 322.15
Description: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

DNA Polymerase alpha Antibody / POLA1 (N-Terminal Region)

F54189-0.05ML 0.05 ml
EUR 140.25
Description: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

DNA Polymerase alpha Antibody / POLA1 (N-Terminal Region)

F54189-0.2ML 0.2 ml
EUR 322.15
Description: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

Anti-DNA polymerase alpha (3C11)

YF-MA14797 100 ug
EUR 435.6
Description: Mouse monoclonal to DNA polymerase alpha

Polymerase DNA Directed Alpha 1 (POLA1) Antibody

abx331254-100ul 100 ul
EUR 510

Polymerase DNA Directed Alpha 1 (POLa1) Antibody

20-abx131487
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Polymerase DNA Directed Alpha 1 (POLa1) Antibody

20-abx131488
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Polymerase DNA Directed Alpha 1 (POLA1) Antibody

20-abx323816
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled

AD-146-U Custom Ask for price

Recombinant (E.Coli) Taq DNA Polymerase

RP-394 1000 IU
EUR 196.8

Polymerase (Dna Directed), alpha 2 (70Kd Subunit) Antibody

20-abx114588
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

DNA Polymerase alpha Blocking Peptide

AF5235-BP 1mg
EUR 234

DNA Polymerase alpha Blocking Peptide

DF3082-BP 1mg
EUR 234

Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated

AD-146-B Custom Ask for price

Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled

AD-146-F Custom Ask for price

DNA Polymerase alpha Cell ELISA Kit

abx595704-96tests 96 tests
EUR 764.4

Polymerase DNA Directed Alpha 1 (POLa1) Polyclonal Antibody

CAU23946-100ul 100ul
EUR 241.9

Polymerase DNA Directed Alpha 1 (POLa1) Polyclonal Antibody

CAU23946-200ul 200ul
EUR 302.4

Polymerase DNA Directed Alpha 1 (POLa1) Polyclonal Antibody

CAU23947-100ul 100ul
EUR 235.2

Polymerase DNA Directed Alpha 1 (POLa1) Polyclonal Antibody

CAU23947-200ul 200ul
EUR 294

Human DNA Polymerase Alpha Assay Kit

HDPA100K 100 assays
EUR 278.4
Description: This product includes all the assay kit components except the enzyme for 100 assays in a 384-well plate assay format: 400 ul of 10 x Buffer, 33 ul of 100 x DNA template, 33 ul of 100 x dNTP mix, 1550 ul of 2 x Dye, 1550 ul of 50 mM EDTA. The kit does not include the enzyme.

Recombinant (E.Coli) Taq Plus DNA Polymerase

RP-395 250 U
EUR 196.8

DNA Polymerase alpha 1 Blocking Peptide

20-abx063100
  • EUR 326.40
  • EUR 493.20
  • 1 mg
  • 5 mg

anti- DNA polymerase beta antibody

FNab02432 100µg
EUR 606.3
Description: Antibody raised against DNA polymerase beta

DNA Polymerase Antibody

E11-11836C 100μg
EUR 225
Description: Available in various conjugation types.

Anti-Taq DNA Polymerase Antibody

48369-100ul 100ul
EUR 399.6

Anti-Taq DNA Polymerase Antibody

48369-50ul 50ul
EUR 286.8

Anti-Taq DNA Polymerase Antibody

E8EM1701-49 100ul
EUR 275
Description: Available in various conjugation types.

Rabbit Anti-Human DNA Polymerase Delta, catalytic subunit (Pol D) antiserum

DNPD11-S 100 ul
EUR 548.4

Human DNA polymerase alpha - for 100 assays

HDPA1-100 100 assays
EUR 309.4
Description: This product includes 7 ul of 4 uM human DNA polymerase alpha catalytic subunit.

Human DNA polymerase alpha - for 1000 assays

HDPA1-1000 100 assays
EUR 1698.95
Description: This product includes 50 ul of 4 uM human DNA polymerase alpha catalytic subunit.

Polymerase DNA Directed Alpha 1 (POLa1) Polyclonal Antibody (Human)

4-PAC713Hu01
  • EUR 296.40
  • EUR 3012.00
  • EUR 750.00
  • EUR 372.00
  • EUR 256.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Polymerase DNA Directed Alpha 1 (POLa1)

Polymerase DNA Directed Alpha 1 (POLa1) Polyclonal Antibody (Mouse)

4-PAC713Mu01
  • EUR 301.20
  • EUR 3091.20
  • EUR 768.00
  • EUR 379.20
  • EUR 258.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Polymerase DNA Directed Alpha 1 (POLa1)

Human DNA Polymerase Alpha Assay Kit Plus

HDPA100KE1 100 assays
EUR 624.67
Description: This product includes all the assay kit components for 100 assays in 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA template, 33 µl of 100 x dNTP mix, 30 µl of 100 x human DNA polymerase alpha (4 µM), 1550 µl of 2 x Dye, 1550 µl of 50 mM EDTA.

Polymerase (DNA Directed), Alpha 2 (70 kDa Subunit) (POLA2) Antibody

abx236605-100ug 100 ug
EUR 661.2

Polymerase (DNA Directed), Alpha 2 (70 kDa Subunit) (POLA2) Antibody

20-abx217845
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

Polymerase (DNA Directed), Alpha 2 (70 kDa Subunit) (POLA2) Antibody

abx036083-100ug 100 ug
EUR 469.2

Polymerase (DNA Directed), Alpha 2 (70 kDa Subunit) (POLA2) Antibody

abx029429-400ul 400 ul
EUR 627.6

Polymerase (DNA Directed), Alpha 2 (70 kDa Subunit) (POLA2) Antibody

abx029429-80l 80 µl
EUR 343.2

Polymerase (DNA Directed), Alpha 2 (70 kDa Subunit) (POLA2) Antibody

20-abx002434
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Polymerase (DNA Directed), Alpha 2 (70 kDa Subunit) (POLA2) Antibody

20-abx306820
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase alpha Cell-Based ELISA Kit

EKA50702-5x96T 5x96T
EUR 2122.78

DNA Polymerase alpha Cell-Based ELISA Kit

EKA50702-96T 96T
EUR 446.9

E. coli DNA polymerase alpha - for 100 assays

DPA-100EC 100 assays
EUR 309.4
Description: This product includes 33 ul of 2 uM E. coli DNA polymerase III alpha.

DNA Polymerase λ Antibody

34098-100ul 100ul
EUR 302.4

DNA Polymerase λ Antibody

34098-50ul 50ul
EUR 224.4

DNA Polymerase β Antibody

33357-100ul 100ul
EUR 302.4

DNA Polymerase β Antibody

33357-50ul 50ul
EUR 224.4

DNA Polymerase ζ Antibody

33602-100ul 100ul
EUR 302.4

DNA Polymerase ζ Antibody

33602-50ul 50ul
EUR 224.4

DNA Polymerase θ Antibody

33611-100ul 100ul
EUR 302.4

DNA Polymerase θ Antibody

33611-50ul 50ul
EUR 224.4

DNA Polymerase α Antibody

33665-100ul 100ul
EUR 302.4

DNA Polymerase α Antibody

33665-50ul 50ul
EUR 224.4

DNA Polymerase β Antibody

E11-0173C 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase ζ Antibody

E11-10144C 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase θ Antibody

E11-10173C 100μg
EUR 225
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E11-10322C 100μg
EUR 225
Description: Available in various conjugation types.

DNA Polymerase β Antibody

E033357 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase ζ Antibody

E033602 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase θ Antibody

E033611 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E033665 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase λ Antibody

E034098 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E19-3082 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E18-5235-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E18-5235-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Polymerase DNA Directed Alpha 1 (POLa1) Polyclonal Antibody (Human), PE

4-PAC713Hu01-PE
  • EUR 355.20
  • EUR 3168.00
  • EUR 900.00
  • EUR 446.40
  • EUR 234.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Polymerase DNA Directed Alpha 1 (POLa1). This antibody is labeled with PE.

Polymerase DNA Directed Alpha 1 (POLa1) Polyclonal Antibody (Mouse), PE

4-PAC713Mu01-PE
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Polymerase DNA Directed Alpha 1 (POLa1). This antibody is labeled with PE.

Anti-Taq DNA Polymerase Antibody [3C3]

EM1701-49 100ul
EUR 210
Description: Polymerase Chain Reaction (PCR), invented by Kary B. Mullis, at the Cetus Corporation, who was awarded the 1993 Nobel Prize for chemistry for PCR, is a technique to exponentially amplify in vitro a small quantity of a specific nucleotide sequence using a thermostable (Taq) DNA polymerase. Anti-Taq Antibody is an ideal tool for hot-start PCR with Taq DNA polymerase. The Anti-Taq Antibody binds to Taq DNA polymerase and arrests the activity of Taq DNA Polymerase, preventing non-specific and primer dimer amplification resulted from non-specific priming at ambient temperature for the duration of time prior to PCR thermal cycling. During the initial denaturing step in PCR thermal cycling, the Anti-Taq Antibody is denatured and the Taq DNA polymerase is then released, thus regaining its full DNA polymerase activity. The result indicates that anti-Taq DNA Polymerase antibody increases the specificity and sensitivity of the PCR.

Polymerase DNA Directed Alpha 1 (POLa1) Polyclonal Antibody (Human), APC

4-PAC713Hu01-APC
  • EUR 414.00
  • EUR 3930.00
  • EUR 1094.40
  • EUR 528.00
  • EUR 262.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Polymerase DNA Directed Alpha 1 (POLa1). This antibody is labeled with APC.

Polymerase DNA Directed Alpha 1 (POLa1) Polyclonal Antibody (Human), Cy3

4-PAC713Hu01-Cy3
  • EUR 502.80
  • EUR 5190.00
  • EUR 1410.00
  • EUR 654.00
  • EUR 301.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Polymerase DNA Directed Alpha 1 (POLa1). This antibody is labeled with Cy3.

Polymerase DNA Directed Alpha 1 (POLa1) Polyclonal Antibody (Human), HRP

4-PAC713Hu01-HRP
  • EUR 379.20
  • EUR 3426.00
  • EUR 968.40
  • EUR 477.60
  • EUR 247.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Polymerase DNA Directed Alpha 1 (POLa1). This antibody is labeled with HRP.

Polymerase DNA Directed Alpha 1 (POLa1) Polyclonal Antibody (Mouse), APC

4-PAC713Mu01-APC
  • EUR 421.20
  • EUR 4038.00
  • EUR 1122.00
  • EUR 538.80
  • EUR 266.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Polymerase DNA Directed Alpha 1 (POLa1). This antibody is labeled with APC.

Polymerase DNA Directed Alpha 1 (POLa1) Polyclonal Antibody (Mouse), Cy3

4-PAC713Mu01-Cy3
  • EUR 512.40
  • EUR 5334.00
  • EUR 1446.00
  • EUR 668.40
  • EUR 304.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Polymerase DNA Directed Alpha 1 (POLa1). This antibody is labeled with Cy3.

Polymerase DNA Directed Alpha 1 (POLa1) Polyclonal Antibody (Mouse), HRP

4-PAC713Mu01-HRP
  • EUR 385.20
  • EUR 3519.60
  • EUR 992.40
  • EUR 486.00
  • EUR 250.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Polymerase DNA Directed Alpha 1 (POLa1). This antibody is labeled with HRP.

Polymerase (DNA Directed), Alpha 2 (70 kDa Subunit) (POLA2) Antibody (HRP)

20-abx306821
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA polymerase beta Antibody

abx232432-100ug 100 ug
EUR 577.2

DNA Polymerase beta Antibody

49605-100ul 100ul
EUR 399.6

DNA Polymerase beta Antibody

49605-50ul 50ul
EUR 286.8

DNA Polymerase beta antibody

70R-31161 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase beta antibody

DNA Polymerase zeta antibody

70R-33711 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase zeta antibody

70R-33727 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase beta antibody

70R-50226 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase beta antibody

Leave a Reply

Your email address will not be published. Required fields are marked *