Polymerases & Mixes

Polymerase chain reaction assay for specific detection of bocavirus-1

Polymerase chain reaction assay for specific detection of bocavirus-1

mytaq

TaqMan-based real-time polymerase chain response assay for particular detection of bocavirus-1 in home cats

Feline bocavirus-1 (FBoV-1) was first found in Hong Kong in 2012, and research have indicated that the virus might trigger feline hemorrhagic enteritis. At the moment, there’s a lack of an efficient and quantitative technique for FBoV-1 detection. On this research, a TaqMan-based quantitative real-time PCR (qPCR) for FBoV-1 detection was established.
Primers and probes have been designed to focus on the conserved area of the FBoV-1 NS1 gene. The sensitivity evaluation indicated that the minimal detection restrict was 4.57 × 101 copies/μL. The specificity take a look at revealed no cross-reaction with seven different widespread feline viruses, together with the identical species-FBoV-2 and FBoV-3. The sensitivity of this technique was 100 instances larger than that of typical PCR (cPCR).
The established technique confirmed good repeatability, with the intra-assay and inter-assay coefficients of variation of 0.18%-1.00% and 0.27%-0.45%, respectively. Moreover, the evaluation of feline feces revealed that the detection fee by qPCR was 7.0% (9/128), whereas that by cPCR was 4.7% (6/128).
In conclusion, the established qPCR assay can quantitatively detect FBoV-1 with a excessive sensitivity, excessive specificity, and good reproducibility, making it a promising approach for the scientific detection of and fundamental and epidemiological analysis on FBoV-1.
Key phrases: Feline bocavirus-1; NS1 gene; Actual-time polymerase chain response; TaqMan.

An assay for DNA polymerase β lyase inhibitors that interact the catalytic nucleophile for binding

DNA polymerase β (Pol β) repairs mobile DNA injury. When such injury is inflicted upon the DNA in tumor cells handled with DNA focused antitumor brokers, Pol β thus diminishes their efficacy. Accordingly, this enzyme has lengthy been a goal for antitumor remedy. Though quite a few inhibitors of the lyase exercise of the enzyme have been reported, none has but confirmed sufficient for improvement as a therapeutic agent.
Within the current research, we developed a brand new technique to determine lyase inhibitors that critically interact the lyase energetic web site major nucleophile Lys72 as a part of the binding interface. This entails a parallel analysis of the impact of the inhibitors on the wild-type DNA polymerase β (Pol β) and Pol β modified with a lysine analogue at place 72.
A mannequin panel of 5 structurally numerous lyase inhibitors recognized in our earlier research (solely one in every of which has been revealed) with unknown modes of binding have been used for testing, and one compound, cis-9,10-epoxyoctadecanoic acid, was discovered to have the specified traits.
This discovering was additional polymerase chain response assay for particular detection an essential electrostatic interplay between the oxygen atom of the epoxy group and Nε of the primary catalytic nucleophile, Lys72.
The technique, which is designed to determine compounds that interact sure structural parts of the goal enzyme, might discover broader utility for identification of ligands with predetermined websites of binding.
Key phrases: Energetic web site interplay; Enzyme modification; Lyase inhibitor; Non-proteinogenic amino acid; Focused inhibitor choice.
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mytaq

DNA Polymerase Mu (POLM) Antibody

abx236614-100g 100 µg
EUR 350

DNA Polymerase Mu (POLM) Antibody

abx323923-100g 100 µg
EUR 250

DNA Polymerase Mu (POLM) Antibody

abx323923-50g 50 µg
EUR 187.5

Polymerase (Dna Directed), Mu Antibody

20-abx114594
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  • 150 ul
  • 50 ul

Polymerase (Dna Directed), Mu Antibody

abx114594-100l 100 µl
EUR 612.5

DNA polymerase mu

E8ER1907-58 100ul
EUR 275
Description: Available in various conjugation types.

DNA Polymerase mu Rabbit Polyclonal Antibody

E10G23360 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Rabbit anti-DNA Polymerase mu Antibody

DL95067A-100ul 100 ul
EUR 270
Description: polmu; DNA-directed DNA/RNA polymerase mu; Pol Mu; Terminal transferase

Rabbit anti-DNA Polymerase mu Antibody

DL95067A-50ul 50 ul
EUR 189
Description: polmu; DNA-directed DNA/RNA polymerase mu; Pol Mu; Terminal transferase

Rabbit anti-DNA Polymerase mu Antibody

YLD2561-100ul 100 ul
EUR 320
Description: Rabbit polyclonal antibody to DNA Polymerase mu

Rabbit anti-DNA Polymerase mu Antibody

YLD2561-50ul 50 ul
EUR 200
Description: Rabbit polyclonal antibody to DNA Polymerase mu

DNA Polymerase Antibody

E11-11836C 100μg
EUR 225
Description: Available in various conjugation types.

DNA Polymerase Antibody

8C10144 50ug
EUR 368
Description: DNA Polymerase ζ Antibody

DNA Polymerase β Antibody

33357 100ul
EUR 319

DNA Polymerase β Antibody

33357-100ul 100ul
EUR 302.4

DNA Polymerase β Antibody

33357-50ul 50ul
EUR 224.4

DNA Polymerase ζ Antibody

33602 100ul
EUR 319

DNA Polymerase ζ Antibody

33602-100ul 100ul
EUR 302.4

DNA Polymerase ζ Antibody

33602-50ul 50ul
EUR 224.4

DNA Polymerase θ Antibody

33611 100ul
EUR 319

DNA Polymerase θ Antibody

33611-100ul 100ul
EUR 302.4

DNA Polymerase θ Antibody

33611-50ul 50ul
EUR 224.4

DNA Polymerase α Antibody

33665 100ul
EUR 319

DNA Polymerase α Antibody

33665-100ul 100ul
EUR 302.4

DNA Polymerase α Antibody

33665-50ul 50ul
EUR 224.4

DNA Polymerase λ Antibody

34098-100ul 100ul
EUR 302.4

DNA Polymerase λ Antibody

34098-50ul 50ul
EUR 224.4

DNA Polymerase β Antibody

E033357 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase ζ Antibody

E033602 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase θ Antibody

E033611 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E033665 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase λ Antibody

E034098 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E19-3082 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E18-5235-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E18-5235-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase ζ Antibody

E11-10144C 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase θ Antibody

E11-10173C 100μg
EUR 225
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E11-10322C 100μg
EUR 225
Description: Available in various conjugation types.

DNA Polymerase β Antibody

E11-0173C 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase λ Antibody

8C11836 50ug
EUR 368
Description: DNA Polymerase λ Antibody

DNA Polymerase β Antibody

8C0173 50ug
EUR 368
Description: DNA Polymerase β Antibody

DNA Polymerase θ Antibody

8C10173 50ug
EUR 368
Description: DNA Polymerase θ Antibody

DNA Polymerase α Antibody

8C10322 50ug
EUR 368
Description: DNA Polymerase α Antibody

DNA Polymerase mu Blocking Peptide

20-abx061844
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  • 1 mg
  • 5 mg

DNA polymerase mu (POLM) rabbit polyclonal antibody, Aff - Purified

AP07620PU-N 50 µg Ask for price

DNA polymerase mu (POLM) rabbit polyclonal antibody, Aff - Purified

R1479P 100 µg Ask for price

DNA Polymerase beta Antibody

35404 100ul
EUR 479

DNA Polymerase beta Antibody

35404-100ul 100ul
EUR 468

DNA Polymerase beta Antibody

E18-0148-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

DNA Polymerase beta Antibody

E18-0148-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase beta Antibody

DF6552 200ul
EUR 420

DNA Polymerase beta Antibody

DF6552-100ul 100ul
EUR 280

DNA Polymerase beta Antibody

DF6552-200ul 200ul
EUR 350

DNA Polymerase zeta Antibody

E38PA4807 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase iota Antibody

E38PA5564 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase beta Antibody

AF0148 200ul
EUR 420

DNA Polymerase beta Antibody

AF0148-100ul 100ul
EUR 280

DNA Polymerase beta Antibody

AF0148-200ul 200ul
EUR 350

DNA Polymerase beta Antibody

49605 100ul
EUR 499

DNA Polymerase beta Antibody

49605-100ul 100ul
EUR 399.6

DNA Polymerase beta Antibody

49605-50ul 50ul
EUR 286.8

DNA Polymerase beta antibody

70R-50226 100 ul
EUR 242
Description: Purified Polyclonal DNA Polymerase beta antibody

DNA Polymerase beta antibody

70R-31161 100 ug
EUR 294
Description: Rabbit polyclonal DNA Polymerase beta antibody

DNA Polymerase zeta antibody

70R-33711 100 ug
EUR 342
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase zeta antibody

70R-33727 100 ug
EUR 294
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA polymerase beta Antibody

abx232432-100ug 100 ug
EUR 577.2

DNA Polymerase beta Antibody

20-abx008852
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  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase beta Antibody

20-abx013060
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  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase beta Antibody

ABF0148 100 ug
EUR 525.6

DNA polymerase beta Antibody

GWB-651A32 0.5 ml Ask for price

DNA polymerase beta Antibody

GWB-C8D577 0.25 ml Ask for price

DNA Polymerase beta Antibody

abx013060-100g 100 µg
EUR 237.5

DNA Polymerase beta Antibody

abx013060-10g 10 µg
EUR 43.75

DNA Polymerase beta Antibody

abx013060-200g 200 µg
EUR 312.5

DNA polymerase beta Antibody

abx232432-100g 100 µg
EUR 350

DNA Polymerase gamma antibody

22870 100ul
EUR 479

DNA Polymerase gamma antibody

22870-100ul 100ul
EUR 468

Fast evaluation of influenza a virus infectivity with a long-range reverse-transcription quantitative polymerase chain response assay.

Background: The polymerase chain response (PCR) is often used to detect viral pathogens due to its excessive sensitivity and specificity. Nonetheless, typical PCR strategies can not decide virus infectivity. Virus infectivity is conventionally examined with strategies such because the plaque assay, regardless that such assays require a number of days.

Lengthy-range reverse-transcription quantitative PCR (RT-qPCR) has beforehand been steered for the fast evaluation of RNA virus infectivity the place the lack of infectivity is attributable to genomic fragmentation.

Strategies: IAV was irradiated with 253.7 nm ultraviolet (UV) rays to induce genomic strand breaks that have been confirmed by a full-length RT-PCR assay. The IAV was then subjected to plaque assay, typical RT-qPCR and long-range RT-qPCR to look at the connection between infectious titer and replica quantity.

A easy linear regression evaluation was carried out to look at the correlation between the outcomes of those assays.

Outcomes: An extended-range RT-qPCR assay was developed and validated for influenza A virus (IAV). Though just a few minutes of UV irradiation was required to utterly inactivate IAV, genomic RNA remained detectable by the standard RT-qPCR and the full-length RT-PCR for NS of viral genome following inactivation.

An extended-range RT-qPCR assay was then designed utilizing RT-priming on the 3′ termini of every genomic section and subsequent qPCR of the 5′ areas. UV-mediated IAV inactivation was efficiently analyzed by the long-range RT-qPCR assay particularly when concentrating on PA of the viral genome. This was additionally supported by the regression evaluation that the long-range RT-qPCR is extremely correlated with plaque assay (Adjusted R2 = 0.931, P = 0.000066).

Conclusions: This research means that IAV infectivity could be predicted with out the infectivity assays. The fast detection of pathogenic IAV has, subsequently, been achieved with this sensing know-how.
Key phrases: Infectivity; Influenza virus; Lengthy-range RT-qPCR; Strand break; Ultraviolet rays; Water remedy.

Genome Polyprotein Polyclonal Antibody

A56743
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  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

Genome Polyprotein Polyclonal Antibody

A68253
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  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

Genome Polyprotein Polyclonal Antibody

A68254
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  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

Genome polyprotein Antibody

20-abx300967
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Genome polyprotein Antibody

1-CSB-PA18549A0Rb
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Dengue virus. This antibody is Unconjugated. Tested in the following application: ELISA

Genome polyprotein Antibody

1-CSB-PA333180LA01HFD
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Hepatitis C virus genotype 1a. This antibody is Unconjugated. Tested in the following application: ELISA

Genome polyprotein Antibody

1-CSB-PA362073LA01HQD
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human rhinovirus A serotype 89. This antibody is Unconjugated. Tested in the following application: ELISA

Genome polyprotein Antibody

CSB-PA4643XA11GSV 100ul / 50ul
EUR 299
Description: Genome polyprotein

Genome polyprotein Antibody

CSB-PA4644XA11GSW 100ul / 50ul
EUR 299
Description: Genome polyprotein

Genome polyprotein Antibody

1-CSB-PA714772LA01HYH
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human enterovirus 71. This antibody is Unconjugated. Tested in the following application: ELISA

Genome Polyprotein Antibody

20-abx109417
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Genome polyprotein Antibody

abx300967-100g 100 µg
EUR 362.5

Genome polyprotein Antibody

abx300967-20g 20 µg
EUR 162.5

Genome polyprotein Antibody

abx300967-50g 50 µg
EUR 250

Genome Polyprotein Antibody

abx109417-100l 100 µl
EUR 162.5

Gag Polyprotein (gag) Antibody

20-abx317799
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Gag Polyprotein (gag) Antibody

20-abx318946
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Gag Polyprotein (GAG) Antibody

abx331332-100ul 100 ul
EUR 510

Gag Polyprotein (gag) Antibody

abx239925-100l 100 µl Ask for price

Gag Polyprotein (gag) Antibody

abx239925-50l 50 µl
EUR 350

Gag Polyprotein (GAG) Antibody

abx375152-96tests 96 tests
EUR 337.5

Gag Polyprotein (gag) Antibody

abx318946-100l 100 µl
EUR 250

Gag Polyprotein (gag) Antibody

abx318946-50l 50 µl
EUR 162.5

Gag Polyprotein (gag) Antibody

abx317799-100g 100 µg
EUR 362.5

Gag Polyprotein (gag) Antibody

abx317799-20g 20 µg
EUR 162.5

Gag Polyprotein (gag) Antibody

abx317799-50g 50 µg
EUR 250

Genome polyprotein Antibody (HRP)

20-abx300968
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Genome polyprotein Antibody (HRP)

20-abx109095
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Genome polyprotein Antibody (HRP)

abx300968-100g 100 µg
EUR 362.5

Genome polyprotein Antibody (HRP)

abx300968-20g 20 µg
EUR 162.5

Genome polyprotein Antibody (HRP)

abx300968-50g 50 µg
EUR 250

Genome polyprotein Antibody (HRP)

abx109095-100l 100 µl
EUR 162.5

Genome polyprotein Antibody (FITC)

20-abx300969
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Genome polyprotein Antibody (FITC)

20-abx107678
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Genome polyprotein Antibody (FITC)

abx300969-100g 100 µg
EUR 362.5

Genome polyprotein Antibody (FITC)

abx300969-20g 20 µg
EUR 162.5

Genome polyprotein Antibody (FITC)

abx300969-50g 50 µg
EUR 250

Genome polyprotein Antibody (FITC)

abx107678-100g 100 µg
EUR 362.5

Genome polyprotein Antibody (FITC)

abx107678-20g 20 µg
EUR 162.5

Genome polyprotein Antibody (FITC)

abx107678-50g 50 µg
EUR 250

Gag Polyprotein (GAG) Antibody (HRP)

20-abx317800
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Gag Polyprotein (GAG) Antibody (HRP)

20-abx318947
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Gag Polyprotein (GAG) Antibody (HRP)

abx318947-100l 100 µl
EUR 250

Gag Polyprotein (GAG) Antibody (HRP)

abx318947-50l 50 µl
EUR 162.5

Gag Polyprotein (GAG) Antibody (HRP)

abx317800-100g 100 µg
EUR 362.5

Gag Polyprotein (GAG) Antibody (HRP)

abx317800-20g 20 µg
EUR 162.5

Gag Polyprotein (GAG) Antibody (HRP)

abx317800-50g 50 µg
EUR 250

Genome polyprotein Antibody (Biotin)

20-abx300970
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Genome Polyprotein Antibody (Biotin)

20-abx105887
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Genome polyprotein Antibody (Biotin)

20-abx106264
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Genome polyprotein Antibody (Biotin)

abx300970-100g 100 µg
EUR 362.5

Genome polyprotein Antibody (Biotin)

abx300970-20g 20 µg
EUR 162.5

Genome polyprotein Antibody (Biotin)

abx300970-50g 50 µg
EUR 250

Genome Polyprotein Antibody (Biotin)

abx105887-100g 100 µg
EUR 362.5

Genome Polyprotein Antibody (Biotin)

abx105887-20g 20 µg
EUR 162.5

Genome Polyprotein Antibody (Biotin)

abx105887-50g 50 µg
EUR 250

Genome polyprotein Antibody (Biotin)

abx106264-100g 100 µg
EUR 362.5

Genome polyprotein Antibody (Biotin)

abx106264-20g 20 µg
EUR 162.5

Genome polyprotein Antibody (Biotin)

abx106264-50g 50 µg
EUR 250

Gag Polyprotein (GAG) Antibody (FITC)

20-abx317801
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Gag Polyprotein (GAG) Antibody (FITC)

20-abx318948
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Gag Polyprotein (GAG) Antibody (FITC)

20-abx107749
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Gag Polyprotein (GAG) Antibody (FITC)

abx107749-100g 100 µg
EUR 362.5

Gag Polyprotein (GAG) Antibody (FITC)

abx107749-20g 20 µg
EUR 162.5

Gag Polyprotein (GAG) Antibody (FITC)

abx107749-50g 50 µg
EUR 250

Gag Polyprotein (GAG) Antibody (FITC)

abx318948-100l 100 µl
EUR 250

Gag Polyprotein (GAG) Antibody (FITC)

abx318948-50l 50 µl
EUR 162.5

Gag Polyprotein (GAG) Antibody (FITC)

abx317801-100g 100 µg
EUR 362.5

Gag Polyprotein (GAG) Antibody (FITC)

abx317801-20g 20 µg
EUR 162.5

Gag Polyprotein (GAG) Antibody (FITC)

abx317801-50g 50 µg
EUR 250

Structural Polyprotein (VP2) Antibody

abx224337-100ug 100 ug
EUR 493.2

Structural Polyprotein (VP2) Antibody

abx224337-100g 100 µg
EUR 362.5

Gag Polyprotein (GAG) Antibody (Biotin)

20-abx317802
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Gag Polyprotein (GAG) Antibody (Biotin)

20-abx318949
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Gag Polyprotein (GAG) Antibody (Biotin)

20-abx106336
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Gag Polyprotein (GAG) Antibody (Biotin)

abx106336-100g 100 µg
EUR 362.5

Gag Polyprotein (GAG) Antibody (Biotin)

abx106336-20g 20 µg
EUR 162.5

Gag Polyprotein (GAG) Antibody (Biotin)

abx106336-50g 50 µg
EUR 250

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